Journal: Molecular medicine (Cambridge, Mass.)

Article Title: Drp1 acetylation mediated by CDK5-AMPK-GCN5L1 axis promotes cerebral ischemic injury via facilitating mitochondrial fission.

doi: 10.1186/s10020-024-00948-y

Figure Lengend Snippet: Fig. 8 Inhibition of AMPK by Compound C protects brain tissues from ischemic damage in mouse models of dMCAO. (A) Representative TTC-stained brain sections and quantitative analysis of infarct volume (n=3). (B) Western blot analysis of p-Drp1 and p-AMPK in brain tissues of dMCAO and control mice treated with or without Compound C (n=3). (C) Immunohistochemistry staining for p-Drp1 and p-AMPK in brain tissues of dMCAO and control mice treated as in (B). Scale bars represent 100 μm (n=3). (D) Acetylated Drp1 was analyzed in brain tissues of dMCAO and control mice treated as in (B) by IP with anti-acetyl lysine antibody and western blotting with anti-Drp1 (n=3). (E) Fluorescence images of MitoSOX staining in brain sections of dMCAO and control mice treated as in (B). Scale bars represent 500 μm (n=3). (F) mtROS levels were analyzed by flow cytometry. Bar graphs show the mtROS levels measured based on fluorescence intensity (n=3). (G) TUNEL staining detected apoptosis in brain tissues of dMCAO and control mice treated as in (B). Scale bars represent 200 μm (n=3). Data are represented as mean ± SD, *P<0.05, **P<0.01, ****P<0.0001, P-value was determined by one-way ANOVA with Dunnett’s post hoc correction

Article Snippet: Compound C (inhibitor of AMPK) (5 μM, MCE, HY-13418 A) was acquired from MCE and dissolved in DMSO.

Techniques: Inhibition, Staining, Western Blot, Control, Immunohistochemistry, Fluorescence, Flow Cytometry, TUNEL Assay

Journal: Cell Reports Medicine

Article Title: CTC-derived pancreatic cancer models serve as research tools and are suitable for precision medicine approaches

doi: 10.1016/j.xcrm.2024.101692

Figure Lengend Snippet:

Article Snippet: Dorsomorphin dihydrochloride , MedChem Express , HY-13418.

Techniques: Recombinant, Blocking Assay, Cell Recovery, Reverse Transcription, Sample Prep, Multiplexing, Membrane, Cell Viability Assay, Software, High Content Screening, Cell Culture

Journal: Analytical and bioanalytical chemistry

Article Title: Simultaneous analysis of buprenorphine, methadone, cocaine, opiates and nicotine metabolites in sweat by liquid chromatography tandem mass spectrometry

doi: 10.1007/s00216-010-4392-2

Figure Lengend Snippet: LCMSMS parameters, retention times (RT) and internal standards (IStd)

Article Snippet: Heroin, nicotine, cotinine, NBUP and EDDP QC samples were prepared from a different lot from Cerilliant, when possible, or from a different vial, with preparation on different days than for calibrators.

Techniques:

Journal: Analytical and bioanalytical chemistry

Article Title: Simultaneous analysis of buprenorphine, methadone, cocaine, opiates and nicotine metabolites in sweat by liquid chromatography tandem mass spectrometry

doi: 10.1007/s00216-010-4392-2

Figure Lengend Snippet: Limits of detection (LOD), limits of quantification (LOQ) and linearity results

Article Snippet: Heroin, nicotine, cotinine, NBUP and EDDP QC samples were prepared from a different lot from Cerilliant, when possible, or from a different vial, with preparation on different days than for calibrators.

Techniques:

Journal: Analytical and bioanalytical chemistry

Article Title: Simultaneous analysis of buprenorphine, methadone, cocaine, opiates and nicotine metabolites in sweat by liquid chromatography tandem mass spectrometry

doi: 10.1007/s00216-010-4392-2

Figure Lengend Snippet: Pooled intra-, inter-, and total imprecision and analytical recovery

Article Snippet: Heroin, nicotine, cotinine, NBUP and EDDP QC samples were prepared from a different lot from Cerilliant, when possible, or from a different vial, with preparation on different days than for calibrators.

Techniques:

Journal: Analytical and bioanalytical chemistry

Article Title: Simultaneous analysis of buprenorphine, methadone, cocaine, opiates and nicotine metabolites in sweat by liquid chromatography tandem mass spectrometry

doi: 10.1007/s00216-010-4392-2

Figure Lengend Snippet: Extraction efficiency, process efficiency and matrix effect

Article Snippet: Heroin, nicotine, cotinine, NBUP and EDDP QC samples were prepared from a different lot from Cerilliant, when possible, or from a different vial, with preparation on different days than for calibrators.

Techniques:

Journal: Analytical and bioanalytical chemistry

Article Title: Simultaneous analysis of buprenorphine, methadone, cocaine, opiates and nicotine metabolites in sweat by liquid chromatography tandem mass spectrometry

doi: 10.1007/s00216-010-4392-2

Figure Lengend Snippet: Drug concentrations in 16 sweat patches worn an average 5.9±2.1 days throughout pregnancy from one opioid-dependent pregnant woman receiving buprenorphine-assisted pharmacotherapy (mean daily dose 15.6 mg/day)

Article Snippet: Heroin, nicotine, cotinine, NBUP and EDDP QC samples were prepared from a different lot from Cerilliant, when possible, or from a different vial, with preparation on different days than for calibrators.

Techniques:

Journal: Journal of Environmental Health Science and Engineering

Article Title: Isolation and identification of a 2,3,7,8-Tetrachlorodibenzo-P-dioxin degrading strain and its biochemical degradation pathway

doi: 10.1007/s40201-021-00626-9

Figure Lengend Snippet: The molecular structure of 2,3,7,8-TCDD

Article Snippet: Fig. 2 Photographs of sample sites at Dagu Drainage River, Tianjin The 2,3,7,8-TCDD standards were purchased from Cerilliant Corporation.

Techniques:

Journal: Journal of Environmental Health Science and Engineering

Article Title: Isolation and identification of a 2,3,7,8-Tetrachlorodibenzo-P-dioxin degrading strain and its biochemical degradation pathway

doi: 10.1007/s40201-021-00626-9

Figure Lengend Snippet: Box-Behnken experimental design and results

Article Snippet: Fig. 2 Photographs of sample sites at Dagu Drainage River, Tianjin The 2,3,7,8-TCDD standards were purchased from Cerilliant Corporation.

Techniques:

Journal: Journal of Environmental Health Science and Engineering

Article Title: Isolation and identification of a 2,3,7,8-Tetrachlorodibenzo-P-dioxin degrading strain and its biochemical degradation pathway

doi: 10.1007/s40201-021-00626-9

Figure Lengend Snippet: Analysis of variance (ANOVA) for the fitted quadratic polynomial model for 2,3,7,8-TCDD degradation

Article Snippet: Fig. 2 Photographs of sample sites at Dagu Drainage River, Tianjin The 2,3,7,8-TCDD standards were purchased from Cerilliant Corporation.

Techniques:

Journal: Journal of Environmental Health Science and Engineering

Article Title: Isolation and identification of a 2,3,7,8-Tetrachlorodibenzo-P-dioxin degrading strain and its biochemical degradation pathway

doi: 10.1007/s40201-021-00626-9

Figure Lengend Snippet: Response surface plot showing the effects of pH and temperature on 2,3,7,8-TCDD degradation by Penicillium sp. QI-1

Article Snippet: Fig. 2 Photographs of sample sites at Dagu Drainage River, Tianjin The 2,3,7,8-TCDD standards were purchased from Cerilliant Corporation.

Techniques:

Journal: Journal of Environmental Health Science and Engineering

Article Title: Isolation and identification of a 2,3,7,8-Tetrachlorodibenzo-P-dioxin degrading strain and its biochemical degradation pathway

doi: 10.1007/s40201-021-00626-9

Figure Lengend Snippet: Response surface analysis contour showing the effects of temperature and pH o 2,3,7,8-TCDD degradation by Penicillium sp. QI-1

Article Snippet: Fig. 2 Photographs of sample sites at Dagu Drainage River, Tianjin The 2,3,7,8-TCDD standards were purchased from Cerilliant Corporation.

Techniques:

Journal: Journal of Environmental Health Science and Engineering

Article Title: Isolation and identification of a 2,3,7,8-Tetrachlorodibenzo-P-dioxin degrading strain and its biochemical degradation pathway

doi: 10.1007/s40201-021-00626-9

Figure Lengend Snippet: Strain were grown in PDA consisting of 1 µg·mL − 1 2,3,7,8-TCDD for 6 days. Values represent the averages of triplicate determinations. Strain growth (▲); 2,3,7,8-TCDD degradation (■)

Article Snippet: Fig. 2 Photographs of sample sites at Dagu Drainage River, Tianjin The 2,3,7,8-TCDD standards were purchased from Cerilliant Corporation.

Techniques:

Journal: Journal of Environmental Health Science and Engineering

Article Title: Isolation and identification of a 2,3,7,8-Tetrachlorodibenzo-P-dioxin degrading strain and its biochemical degradation pathway

doi: 10.1007/s40201-021-00626-9

Figure Lengend Snippet: The Degradation rate of 2,3,7,8-TCDD by 2,3,7,8--TCDD -degrading strain after 6 days

Article Snippet: Fig. 2 Photographs of sample sites at Dagu Drainage River, Tianjin The 2,3,7,8-TCDD standards were purchased from Cerilliant Corporation.

Techniques: Concentration Assay

Journal: Journal of Environmental Health Science and Engineering

Article Title: Isolation and identification of a 2,3,7,8-Tetrachlorodibenzo-P-dioxin degrading strain and its biochemical degradation pathway

doi: 10.1007/s40201-021-00626-9

Figure Lengend Snippet: The degradation curve of 2,3,7,8-TCDD during 6days

Article Snippet: Fig. 2 Photographs of sample sites at Dagu Drainage River, Tianjin The 2,3,7,8-TCDD standards were purchased from Cerilliant Corporation.

Techniques:

Journal: Journal of Environmental Health Science and Engineering

Article Title: Isolation and identification of a 2,3,7,8-Tetrachlorodibenzo-P-dioxin degrading strain and its biochemical degradation pathway

doi: 10.1007/s40201-021-00626-9

Figure Lengend Snippet: Proposed pathway of 2,3,7,8-TCDD degradation by Penicillium sp. QI-1

Article Snippet: Fig. 2 Photographs of sample sites at Dagu Drainage River, Tianjin The 2,3,7,8-TCDD standards were purchased from Cerilliant Corporation.

Techniques:

Journal: American Journal of Translational Research

Article Title: ER stress induces myocardial dysfunction and cardiac autophagy in Sestrin2 knockout mice

doi:

Figure Lengend Snippet: Effect of tunicamycin on cell shortening in cardiomyocytes from C57 mice. Cardiomyocytes were incubated with tunicamycin (3 μg/ml) for 4 h in vitro prior to assessment of mechanical properties. A cohort of cardiomyocytes was coincubated with the AMPK inhibitor compound C (10 mM) or the autophagy inhibitor 3-methyladenine (3-MA, 3 mM) alone with TN. A: Resting cell length; B: Peak shortening (PS, normalized to cell length); C: Maximal velocity of shortening (+dL/dt); D: Maximal velocity of relengthening (-dL/dt); E: Time-to-PS (TPS); F: Time-to-90% relengthening (TR90). Mean ± SEM, 3-4 mice per group, *P < 0.05 vs. C57 group; #P < 0.05 vs. TN group.

Article Snippet: Cardiomyocytes were next administered vehicle (DMSO), tunicamycin (3 μg/mL), Compound C (10 μM; Enzo Life Sciences, USA) and 3-methyladenine (3-MA, 3 mM; Selleckchem, USA) for 4 h.

Techniques: Incubation, In Vitro